The Xist lncRNA exploits three-dimensional genome architecture to spread across the X chromosome. Expansion microscopy with conventional antibodies and fluorescent proteins. Expansion microscopy of biological specimens with protein retention. RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. The local transcriptome in the synaptic neuropil revealed by deep sequencing and high-resolution imaging. Next-generation in situ hybridization chain reaction: higher gain, lower cost, greater durability. Programmable in situ amplification for multiplexed imaging of mRNA expression. Imaging individual mRNA molecules using multiple singly labeled probes. Raj, A., van den Bogaard, P., Rifkin, S.A., van Oudenaarden, A. Fluorescence in situ hybridization: past, present and future. Visualization of single RNA transcripts in situ. ExFISH thus enables super-resolution imaging of RNA structure and location with diffraction-limited microscopes in thick specimens, such as intact brain tissue and other tissues of importance to biology and medicine.Ĭhen, F., Tillberg, P.W. Expansion FISH (ExFISH) separates RNAs and supports amplification of single-molecule signals (i.e., via hybridization chain reaction) as well as multiplexed RNA FISH readout. Then, postexpansion, fluorescent in situ hybridization (FISH) imaging of RNA can be performed with high yield and specificity as well as single-molecule precision in both cultured cells and intact brain tissue. We developed a small-molecule linker that enables RNA to be covalently attached to a swellable polyelectrolyte gel synthesized throughout a biological specimen. Here, we present a strategy for expansion microscopy of RNA. The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in normal and pathological biological settings.
0 kommentar(er)
